Genetic evolution of HCV quasispecies in patients with cholestatic h... - SRA

SRX4911920: Genetic evolution of HCV quasispecies in patients with cholestatic hepatitis after liver transplantation
1 LS454 (454 GS Junior) run: 93 spots, 31,620 bases, 60,862b downloads

Design: The aim of this study was to analyze HCV quasispecies in patients with cholestatic hepatitis C (compared to a control group) by deep pyrosequencing. To this end, we included 22 HCV-infected patients undergoing liver transplantation: 13 patients with a diagnosis of cholestatic hepatitis C and 9 patients with mild hepatitis C recurrence (control group). Two serum samples were collected from each patient: one sample obtained at the time of LT and another sample obtained between 1 and 3 months after LT at the time of diagnosis of acute hepatitis. We amplified a 340-nucleotide (nt) fragment of the NS5B region (nt 8279 to 8618, according to isolate H77, accession number AF009606). Deep pyrosequencing was performed using the 454/GS-Junior platform.

Submitted by: CIBERehd, Hospital Clinic, IDIBAPS

Study: Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after transplantation

PRJNA493793 • SRP166308 • All experiments • All runs

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Cholestatic hepatitis C (CHC) is a severe form of HCV infection recurrence that leads to high graft loss rates early after liver transplantation (LT). To investigate the pathogenic mechanisms of CHC, we analyzed HCV quasispecies in patients with CHC compared to a control group (mild hepatitis C recurrence) by deep pyrosequencing.

Sample:

SAMN10256244 • SRS3957516 • All experiments • All runs

Organism: Hepacivirus hominis

Library:

Name: T081_PRE

Instrument: 454 GS Junior

Strategy: AMPLICON

Source: VIRAL RNA

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 93 spots, 31,620 bases, 60,862b

Run	# of Spots	# of Bases	Size	Published
SRR8084942	93	31,620	60,862b	2018-10-23

ID:: 6613280

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